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RNase III is required for localization to the nucleoid of the 5' pre-rRNA leader and for optimal induction of rRNA synthesis in E. coli.
Malagon F
RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 Jul 26. PubMed Article

Plasmids from Article

ID Plasmid Purpose
51557pBAD24-fisE. coli fis ORF cloned into pBAD24 expression vector
51558pBAD24-sfGFPx1Superfolder GFP ORF cloned into pBAD24 for expression in E. coli
51559pBAD24-sfGFPx2Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
51560pBAD24-rnc-sfGFPGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).

Antibodies from Article