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pBAD24-sfGFPx2
(Plasmid #51559)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51559 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Maintained in DH5alpha, assayed in MG1655 or BW25113 after retransformation (fluorescence intensity for this plasmid is background dependent).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    superfolder GFP
  • Species
    Synthetic
  • Mutation
    Contains additonal aa in the N and C terminus (in frame with the polylinker)
  • Tags / Fusion Proteins
    • MARARA (N terminal on insert)
    • GLESTCRHASLAVLADERRFSA (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sfGFP in this plasmid contains Y148C and K214I mutations that do not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD24-sfGFPx2 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51559 ; http://n2t.net/addgene:51559 ; RRID:Addgene_51559)
  • For your References section:

    RNase III is required for localization to the nucleoid of the 5' pre-rRNA leader and for optimal induction of rRNA synthesis in E. coli. Malagon F. RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 Jul 26. 10.1261/rna.038588.113 PubMed 23893733