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Addgene

pBAD24-rnc-sfGFP
(Plasmid #51560)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51560 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBAD24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Maintained in DH5alpha, assayed in MG1655 after retransformation.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rnc
  • Alt name
    RNase III
  • Species
    E. coli
  • Mutation
    Contains additonal aa at the end of the sfGFP in the N and C terminus (in frame with the polylinker)
  • Entrez Gene
    rnc (a.k.a. b2567, ECK2565)
  • Tags / Fusion Proteins
    • superfolder GFP (C terminal on insert)
    • GLESTCRHASLAVLADERRFSA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD24-rnc-sfGFP was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51560 ; http://n2t.net/addgene:51560 ; RRID:Addgene_51560)
  • For your References section:

    RNase III is required for localization to the nucleoid of the 5' pre-rRNA leader and for optimal induction of rRNA synthesis in E. coli. Malagon F. RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 Jul 26. 10.1261/rna.038588.113 PubMed 23893733