Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37234||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7474
Vector typeBacterial Expression
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
Entrez GenePAB1 (a.k.a. YER165W)
- Promoter T7
/ Fusion Protein
- intein (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site SmaI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTYB2-PAB1 was a gift from Jon Lorsch (Addgene plasmid # 37234 ; http://n2t.net/addgene:37234 ; RRID:Addgene_37234)
For your References section:Multiple elements in the eIF4G1 N-terminus promote assembly of eIF4G1*PABP mRNPs in vivo. Park EH, Walker SE, Lee JM, Rothenburg S, Lorsch JR, Hinnebusch AG. EMBO J. 2011 Jan 19;30(2):302-16. Epub 2010 Dec 7. 10.1038/emboj.2010.312 PubMed 21139564