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Plasmid#124947PurposeExpression of RFP-TEVproteaseDepositorInsertRFP-TEVprotease
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM5-Flag
Plasmid#180410Purposelentiviral transduction of human TGM5 (with a C-terminal Flag tag) geneDepositorInsertTGM5 (TGM5 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro1 T18N
Plasmid#47890PurposeExpresses myc tagged Miro1 T18N dominant negative mutantDepositorInsertMiro1 T18N (RHOT1 Human)
UseTagsmycExpressionMammalianMutationT18N, dominant negative mutantPromoterCMVAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_ActB_MS2
Plasmid#81083PurposeTranslation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_ActB_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4-GFP-TEV-B55
Plasmid#217378Purposeexpresses GFP tagged B55 in mammalian cells; tev cleavableDepositorInsertB55 (PPP2R2A Human)
UseTagsEGFP TEVExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx-HTa-SOD1 WT
Plasmid#191849Purposeexpress wild-type SOD1 in bacterial cellsDepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-kz-CD20-Puro
Plasmid#209759PurposeLentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK3_038
Plasmid#123752PurposeEncodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK systemDepositorInsertR-FlincA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPL5608_TEF1*-Cre_TRP1
Plasmid#60931PurposeConstitutive expression vector for Cre-recombinase containing TRP1 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromoterMutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PH-PLC-delta-EGFP
Plasmid#136998PurposeLentiviral EGFP lipid sensor for PI(4,5)P2DepositorInsertPH-PLC-delta-EGFP
UseLentiviralTagsEGFPExpressionMutationPromoterEF1 alphaAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExchange_tdk_BT1311p-tetR-1
Plasmid#90325PurposeSuicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702DepositorInsertTetR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGabrg2
Plasmid#124857PurposeMutagenesis of Gabrg2DepositorInsertGabrg2 (Gabrg2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorInsertLDLR (LDLR Human)
UseTagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
SP-Cas9
Plasmid#62731PurposeExpresses SP-Cas9 protein in bacterial cellsDepositorInsertSP-CAS9
UseCRISPRTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF1771
Plasmid#143542PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTBP (TBP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
STIM1-EGFP
Plasmid#210370PurposeExpresses STIM1 fused with EGFP in mammalian cellsDepositorInsertSTIM1 (STIM1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasAb-apr
Plasmid#121998PurposeBacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumanniiDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
HOP-flash
Plasmid#83467PurposeTEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only