165,744 results

1. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6 and H1

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. NLS-GFP-iLID-Ferritin

   Plasmid

   #227376

   Purpose

   Condensate module, light-inducible oligomerization 'core'

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   GFP 'core'
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

   Plasmid

   #190111

   Purpose

   AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Use

   AAV

   Tags

   bpNLS

   Mutation

   H840A

   Promoter

   EF1a

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-YFP

   Plasmid

   #13033

   Purpose

   Mammalian expression vector for cloning your gene fused to YFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Yellow fluorescent protein
   

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. AID-mTagBFP2-loxP_myo2_neoR-loxP

   Plasmid

   #194055

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Tags

   codon optimized mTagBFP2

   Expression

   Worm

   Promoter

   None

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-EF1a ANXA11-mEmerald

   Plasmid

   #164210

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Promoter

   EF1a

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLminP_Luc2P_RE34

   Plasmid

   #90376

   Purpose

   MAPK - Elk1 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   ELK1

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV_EF1a_CDK4-P2A-Hygro_Barcode

   Plasmid

   #170223

   Purpose

   Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYIC

   Plasmid

   #18673

   Depositor

   Han Htun

   Article

   Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

   Type

   Empty backbone

   Tags

   3Myc-EYFP-HA-His6 and ECFP-HA-His6

   Expression

   Mammalian

   Available Since

   Aug. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1 hSCN5A-L325R

    Plasmid

    #145373

    Purpose

    expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Expression

    Mammalian

    Mutation

    changed Leucine 325 to Arginine

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDM1047

    Plasmid

    #108990

    Purpose

    Inducible expression vector (dox-on), N-terminal GFP tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Dictyostelium discoideum inducible expression vec…

    Tags

    GFP

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-EGFP-2xFYVE

    Plasmid

    #136996

    Purpose

    Lentiviral EGFP lipid sensor for PI(3)P

    Depositor

    Ken-Ichi Takemaru

    Insert

    EGFP-2xFYVE
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    EF1 alpha

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF1676

    Plasmid

    #143832

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUNX1T1 (RUNX1T1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet-O-FUW-Neurod1

    Plasmid

    #30129

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    NeuroD1 (Neurod1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. mClY-N1

    Plasmid

    #90457

    Purpose

    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

    Insert

    mClY-N1
    

    Expression

    Mammalian

    Mutation

    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK651

    Plasmid

    #71702

    Purpose

    Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-XPO1-E571K

    Plasmid

    #116708

    Purpose

    Lentiviral expression of XPO1 E571K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Lentiviral

    Mutation

    E571K

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6-1 and dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TRE3G-MYCN

    Plasmid

    #104542

    Purpose

    PiggyBac vector encoding doxycycline-inducible human MYCN

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
    

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO-FOXM1-shRNA-84

    Plasmid

    #89494

    Purpose

    Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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