161,641 results

1. BoxB in pcDNA3

   Plasmid

   #29727

   Depositor

   Howard Chang

   Article

   Wang et al Nature. 2011 Mar 20. ():.

   Insert

   5BoxB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEmerald-Sec61b-C1

   Plasmid

   #90992

   Purpose

   Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

   Insert

   mEmerald-Sec61b (SEC61B Human, Synthetic)
   

   Use

   Tags

   mEmerald (green fluorescence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG dCas9-KRAB-2A-EGFP

   Plasmid

   #92396

   Purpose

   CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9_KRAB_2A_EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mApple-TOMM20-N-10

   Plasmid

   #54955

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b-12x

   Plasmid

   #61699

   Purpose

   Expresses PTDH mutant Opt12x

   Depositor

   Huimin Zhao

   Article

   Johannes et al Biotechnol Bioeng. 2007 Jan 1;96(1):18-2

   Insert

   Opt12x
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

6. TPC2-G-GECO1.2

   Plasmid

   #207141

   Purpose

   Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPC2N2 (TPCN2 Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TPC1-G-GECO1.2

   Plasmid

   #207143

   Purpose

   Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   TPCN1 (TPCN1 Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

   Plasmid

   #100835

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLIK-Rbfox2-rABE-Flag

    Plasmid

    #191384

    Purpose

    Doxycycline inducible expression of Rbfox2-rABE

    Depositor

    Stephen Floor

    Article

    Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

    Insert

    Rbfox2-rABE (RBFOX2 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    tet-inducible promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRY2high-mCherry

    Plasmid

    #104063

    Purpose

    Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2high-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CRY2PHR E490R

    Promoter

    CMV

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N2-COL1A1

    Plasmid

    #66602

    Purpose

    Expresses procollagen 1 alpha 1 as a GFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-cTNT-Cre

    Plasmid

    #209785

    Purpose

    Expresses Cre by the specific cTNT promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    cTNT

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUGW-FIRE-pHLy

    Plasmid

    #170774

    Purpose

    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    hUbC

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lamp1_mStayGold

    Plasmid

    #221902

    Purpose

    Expresses mStayGold fused to Lamp1

    Depositor

    Janelia Integrative Imaging

    Insert

    Lamp1_mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-miniTurbo

    Plasmid

    #124647

    Purpose

    Expresses Flag-miniTurbo fusion for proximity labeling

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMH-Halo tag

    Plasmid

    #154144

    Purpose

    Gateway compatible mammalian expression plasmid for halo tag fusion proteins

    Depositor

    Michael Huen

    Type

    Empty backbone

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE Flag HA ADAR1 p150

    Plasmid

    #237855

    Purpose

    Lentiviral expression of ADAR1 p150

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    ADAR1 (ADAR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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