pCI-neo Fluc EGFP
PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90170||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5472
- Total vector size (bp) 7860
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameFLuc EGFP
Insert Size (bp)2373
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCI-neo Fluc EGFP was a gift from Franz-Ulrich Hartl (Addgene plasmid # 90170 ; http://n2t.net/addgene:90170 ; RRID:Addgene_90170)
For your References section:Firefly luciferase mutants as sensors of proteome stress. Gupta R, Kasturi P, Bracher A, Loew C, Zheng M, Villella A, Garza D, Hartl FU, Raychaudhuri S. Nat Methods. 2011 Sep 4;8(10):879-84. doi: 10.1038/nmeth.1697. 10.1038/nmeth.1697 PubMed 21892152