Purpose(Empty Backbone) All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80474||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3000
Vector typeMammalian Expression ; piggyBac transposon
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
- Cloning method Gateway Cloning
Requires co-transfection with a piggyBac transposase plasmid for stable genomic integration. If you desire to use transposase with the Materials, you may contact Transposagen or other licensed vendor.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PB-TA-ERN was a gift from Knut Woltjen (Addgene plasmid # 80474 ; http://n2t.net/addgene:80474 ; RRID:Addgene_80474)
For your References section:Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen K. Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251. 10.1007/7651_2015_251 PubMed 26025620