pcDNA5/FRT/TO-neo (1795)
(Plasmid #41000)

• Purpose: (Empty Backbone)
• Depositing Lab: Jonathon Pines
• Publication: Jonathon Pines lab pcDNA5/FRT/TO-based cloning vectors (unpublished)

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Modifications to backbone: HygroR replaced with NeoR
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Hygromycin resistance cassette in pcDNA5/FRT/TO has been replaced with the Neomycin resistance casette from pEGFP-N1.

This plasmid can be used to create inducible stable cell lines with the FRT T-Rex system in RPE cells (RPE cells are Hygromycin resistant due to immortalization with telomerase and therefore unmodified pcDNA5/FRT/TO (originally conferring Hygromycin resistance) cannot be used.).