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pcDNA5/FRT/TO-neo (1795)
(Plasmid #41000)


Item Catalog # Description Quantity Price (USD)
Plasmid 41000 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Modifications to backbone
    HygroR replaced with NeoR
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Hygromycin resistance cassette in pcDNA5/FRT/TO has been replaced with the Neomycin resistance casette from pEGFP-N1.

This plasmid can be used to create inducible stable cell lines with the FRT T-Rex system in RPE cells (RPE cells are Hygromycin resistant due to immortalization with telomerase and therefore unmodified pcDNA5/FRT/TO (originally conferring Hygromycin resistance) cannot be used.).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO-neo (1795) was a gift from Jonathon Pines (Addgene plasmid # 41000 ; ; RRID:Addgene_41000)