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pcDNA5/FRT/TO-Venus-Flag (865)
(Plasmid #40998)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 40998 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    Venus YFP-3xFlag inserted via BstXI-XhoI sites
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Hygromycin
  • Tags / Fusion Proteins
    • Venus YFP (C terminal on insert)
    • 3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Venus YFP-3xFlag was inserted into this vector via BstXI-XhoI sites.

This vector can be used to express proteins fused to Venus YFP with a C-terminal 3xFlag tag.

The MCS consists of PmeI (non-unique), AflII, HindIII, Asp718I, KpnI, BamHI, and BstXI upstream of Venus. Additionally, the following sites are present after the Venus-3xFlag sequence: XhoI, Eco0109I, ApaI, PmeI (non-unique), and BclI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO-Venus-Flag (865) was a gift from Jonathon Pines (Addgene plasmid # 40998 ; http://n2t.net/addgene:40998 ; RRID:Addgene_40998)