Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40998||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneVenus YFP-3xFlag inserted via BstXI-XhoI sites
Vector typeMammalian Expression
- Promoter CMV
/ Fusion Proteins
- Venus YFP (C terminal on insert)
- 3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) (C terminal on insert)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer LNCX
- 3′ sequencing primer EGFP-N, BGH-rev (Common Sequencing Primers)
Venus YFP-3xFlag was inserted into this vector via BstXI-XhoI sites.
This vector can be used to express proteins fused to Venus YFP with a C-terminal 3xFlag tag.
The MCS consists of PmeI (non-unique), AflII, HindIII, Asp718I, KpnI, BamHI, and BstXI upstream of Venus. Additionally, the following sites are present after the Venus-3xFlag sequence: XhoI, Eco0109I, ApaI, PmeI (non-unique), and BclI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA5/FRT/TO-Venus-Flag (865) was a gift from Jonathon Pines (Addgene plasmid # 40998 ; http://n2t.net/addgene:40998 ; RRID:Addgene_40998)