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pCMV-T7 RNA POL

Plasmid

#138352

Purpose

Expressing T7 RNA Polymerase in mammalian cells

Depositor

Baisong Lu

Insert

T7 RNA POL

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

Plasmid

#194063

Purpose

Mammalian expression of evoCjCas9 PEmax_deltaRnH

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-PEmax_dRnH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2w/b

Plasmid

#47586

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b (ATP2B2 Human)

Use

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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myc-KIF5C

Plasmid

#127618

Purpose

N-terminal myc-tagged mouse KIF5C

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5C (Kif5c Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

Plasmid

#168998

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Unspecified

Tags

EGFP and mRFP1

Expression

Mutation

Promoter

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-DD-GFP

Plasmid

#187260

Purpose

Lentiviral expression of myosin regulatory light chain-DD mutant

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

T18D, S19D

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-AA-GFP

Plasmid

#187259

Purpose

Lentiviral expression of myosin regulatory light chain-AA mutant

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

T18A,S19A

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Green Glifon600

Plasmid

#126207

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon600

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMOS028: Mitycam calcium sensor (mitochondrial)

Plasmid

#163046

Purpose

Mitycam calcium sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Mitycam

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Flamindo2

Plasmid

#73938

Purpose

Expresses biosensor for cAMP in mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

Insert

Flamindo2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-HsNRF2 (NFE2L2)

Plasmid

#194303

Purpose

Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

NFE2L2 (NFE2L2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Plxna1-Fc-His

Plasmid

#72122

Purpose

Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna1 (Plxna1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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SP-Cas9

Plasmid

#62731

Purpose

Expresses SP-Cas9 protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

SP-CAS9

Use

CRISPR

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3)P Probe

Plasmid

#211508

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

FYVE-Hrs x 2 (PIKFYVE Human)

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTCF236 HLA-B*40:01

Plasmid

#180463

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

HLA-B*40:01

Use

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Promoter

Available since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mNG

Plasmid

#183460

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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PM-B

Plasmid

#209876

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Use

Tags

Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-puro-cOVA

Plasmid

#135073

Purpose

Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance

Depositor

Maria Castro

Insert

cOVA (OVAL Chicken)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 BRE Luciferase

Plasmid

#45126

Depositor

Peter ten Dijke, Martine Roussel

Article

Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

Insert

Id1-BRE

Use

Luciferase; Bmp/smad transcriptional reporter

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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