159,461 results

1. pOT_2 - lenti-EFS-tNGFR-2A-puro

   Plasmid

   #181971

   Purpose

   Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   tNGFR (NGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   truncation: aa 11-276

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDA_886

   Plasmid

   #201162

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. eGFP-hCOL1A1

   Plasmid

   #140110

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag, K20…

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ shNS

   Plasmid

   #127696

   Purpose

   Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shNS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSI2-ADA

   Plasmid

   #166967

   Purpose

   Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

   Depositor

   Michael Kharas

   Article

   Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

   Insert

   MSI2-ADA
   

   Use

   Retroviral

   Tags

   MSI2-ADA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSK664

   Plasmid

   #205130

   Purpose

   Expression of GFP-2xPX (from SnxA) in mammalian cells

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   tandem 2xPX domain of SnxA
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pF2-DTA

   Plasmid

   #42521

   Depositor

   Terry Magnuson

   Article

   Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

   Insert

   EF1a-DTA
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-CMV-NES-jRGECO1a

   Plasmid

   #61563

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRGECO1a
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Casp8

   Plasmid

   #11817

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

   Insert

   Caspase 8 (CASP8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. IST1-GFP

    Plasmid

    #186298

    Purpose

    Expresses human IST1 C-terminally fused to GFP

    Depositor

    Geert van den Bogaart

    Article

    Stempels et al J Cell Biol. 2023 Jul 3;222(7):e20220513

    Insert

    IST1 homolog (IST1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    GFP

    Promoter

    CMV

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCAR_Cas9-blast_GFP

    Plasmid

    #162074

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-blasticidin S deaminase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCX419

    Plasmid

    #229216

    Purpose

    All-in-one plasmid for labeling of XIST RNA via Csm complex

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-Trastuzumab-IgG1/κ

    Plasmid

    #61883

    Purpose

    Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMKO.1 puro

    Plasmid

    #8452

    Purpose

    Retroviral vector for shRNA expression.

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. CaMKIIP-mEGFP-P2A-paAIP2/pAAV

    Plasmid

    #91718

    Purpose

    AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

    Insert

    mEGFP-P2A-paAIP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII 1.3

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-IP- SCAT3.2

    Plasmid

    #172497

    Purpose

    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Amcyan
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    MLV LTR

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBTK522-FAST-PETase

    Plasmid

    #214754

    Purpose

    plasmid for expression FAST-PETase in Pseudomonas putida

    Depositor

    Hal Alper

    Article

    Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

    Insert

    FAST-PETase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWM_12x601_30bpLinker

    Plasmid

    #157789

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_30bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GalT-RpHLuorin2

    Plasmid

    #171719

    Purpose

    Trans-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV_DENV4_PrME

    Plasmid

    #207266

    Purpose

    Mammalian expression of Dengue virus 4 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV4_PrME (POLY Dengue virus 4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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