Programmable RNA Tracking in Live Cells with CRISPR/Cas9.
Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, Yeo GW
Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 10.1016/j.cell.2016.02.054.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74705 | pBluescriptSKII+ U6-sgRNA(F+E) ACTB | Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA |
| 74707 | pBluescriptSKII+ U6-sgRNA(F+E) empty | Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence |
| 74709 | pBluescriptSKII+ U6-sgRNA(F+E) TFRC | Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA |
| 74710 | pCDNA3.1- dCas9-2xNLS-EGFP | Expresses dCas9-2xNLS-EGFP in mammalian cells |