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pBluescriptSKII+ U6-sgRNA(F+E) TFRC
(Plasmid #74709)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 74709 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
  • gRNA/shRNA sequence
    TFRC 3'UTR
  • Promoter U6

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescriptSKII+ U6-sgRNA(F+E) TFRC was a gift from Eugene Yeo (Addgene plasmid # 74709 ; ; RRID:Addgene_74709)
  • For your References section:

    Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, Yeo GW. Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 10.1016/j.cell.2016.02.054. 10.1016/j.cell.2016.02.054 PubMed 26997482