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pBluescriptSKII+ U6-sgRNA(F+E) empty
(Plasmid #74707)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74707 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    pBlueScript II SK(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
  • gRNA/shRNA sequence

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescriptSKII+ U6-sgRNA(F+E) empty was a gift from Eugene Yeo (Addgene plasmid # 74707 ; ; RRID:Addgene_74707)
  • For your References section:

    Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, Yeo GW. Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 10.1016/j.cell.2016.02.054. 10.1016/j.cell.2016.02.054 PubMed 26997482