PurposeAn AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||126462||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7914
- Total vector size (bp) 5758
Vector typeMammalian Expression, AAV
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameL7-6, Purkinje cell specific promoter
Alt namepcp2 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)844
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer L7-6-rev, GGGGAGGGGGAGCAGATATC
- 3′ sequencing primer GFP-rev, ACTTGTGGCCGTTTACGTCGC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe gene origin of L7-6 promoter is L7-4 promoter that is described in the paper (Nitta et al, 2017).
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that the Addgene verified sequence contains a truncated ITR compared to the depositor's reference sequence. This truncation was present in the original stock and still worked well for AAV packaging.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV/L7-6-GFP-WPRE was a gift from Hirokazu Hirai (Addgene plasmid # 126462 ; http://n2t.net/addgene:126462 ; RRID:Addgene_126462)
For your References section:Minimal Purkinje Cell-Specific PCP2/L7 Promoter Virally Available for Rodents and Non-human Primates. Nitta K, Matsuzaki Y, Konno A, Hirai H. Mol Ther Methods Clin Dev. 2017 Jul 27;6:159-170. doi: 10.1016/j.omtm.2017.07.006. eCollection 2017 Sep 15. S2329-0501(17)30089-X [pii] PubMed 28828391