165,750 results

1. pAIDFA-EF1a-NmScarlet-mAID

   Plasmid

   #140615

   Purpose

   Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1 mutated F74A
   

   Expression

   Mammalian

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

   Plasmid

   #201684

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1 (V106W)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pp-mCAT1

   Plasmid

   #83948

   Purpose

   This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

   Depositor

   Vincent Lotteau, Philippe Mangeot

   Article

   Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

   Insert

   mCAT1 (Slc7a1 )
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGRG36-Kn-PA1-GFP

   Plasmid

   #79088

   Purpose

   pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

   Depositor

   Frank Rosenzweig

   Article

   Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

   Insert

   GFP
   

   Use

   Bacterial chromosome integration and expression o…

   Expression

   Bacterial

   Promoter

   PA1

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAMA-empty

   Plasmid

   #74755

   Purpose

   Mammalian lentiviral vector

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_His-TEV-EiCsm6

   Plasmid

   #172487

   Purpose

   Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

   Insert

   Enterococcus italicus Csm6 (codon optimized)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7 promoter and lac operator

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HPSE-bio-His

   Plasmid

   #53407

   Purpose

   Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   HPSE (HPSE Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223-LRPPRC

   Plasmid

   #23521

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   LRPPRC (LRPPRC Human)
   

   Use

   Gateway donor vector

   Mutation

   G246A; deleted E579

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV8R-12 Capsid

   Plasmid

   #213968

   Purpose

   AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Type

   Empty backbone

   Use

   AAV

   Available Since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-hSTING (N154S)

    Plasmid

    #214160

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (N154S) (STING1 Human)
    

    Expression

    Mammalian

    Mutation

    N154S

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Split 'n coexpress CUL3-RBX1

    Plasmid

    #52293

    Purpose

    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Inserts

    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    

    Tags

    GST, IRES, and TEV

    Expression

    Bacterial

    Mutation

    contains aa 1-309, contains aa 310-768, and conta…

    Promoter

    IRES and tac

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. 7xSMAD_RE::FLuc

    Plasmid

    #124531

    Purpose

    SMAD2 FLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    SMAD FLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti6-DEST PINK1-V5 WT

    Plasmid

    #13320

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCVII-LO

    Plasmid

    #162754

    Purpose

    Retroviral (MSCV) expression of LSSmOrange fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    LSSmOrange
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos4b-TOMM20-N-10

    Plasmid

    #57518

    Purpose

    Localization: Mitochondrial Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson, Loren Looger

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mEos4b

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. human ACF

    Plasmid

    #112860

    Purpose

    plasmid to overexpress human APOBEC1-complementation factor (ACF; A1CF) in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 complementation factor (A1CF Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEGFP-PI35P2

    Plasmid

    #92419

    Purpose

    Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

    Insert

    MCOLN1 (Mcoln1 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Synthetic gene, codon optimized for human.

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXR004: CasRx pre-gRNA cloning backbone

    Plasmid

    #109054

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

    Plasmid

    #230989

    Purpose

    Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Membrane-localized Voltron2 and soma-localized CheRiff
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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