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pAC-BETAipi

Plasmid

#53277

Purpose

Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

idi

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEF5-FRT-TagRFP-T-IFT88

Plasmid

#61684

Purpose

Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

Depositor

Maxence Nachury

Publication

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IFT88 (Ift88 Mouse)

Tags

TagRFP.T

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

Plasmid

#60815

Purpose

dCas9 with single cysteine residue (M1C) for site-specific labelling

Depositor

Jennifer Doudna

Publication

O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

Insert

dCas9 M1C D10A C80S H840A C574S

Tags

His10 and MBP

Expression

Bacterial

Mutation

M1C D10A C80S H840A C574S

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

Plasmid

#50454

Purpose

Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

Depositor

Bryan Roth

Has service

AAV8

Insert

hM3D(Gq)-IRES-mCitrine (CHRM3 Human)

Use

AAV

Tags

HA

Mutation

See supplemental documents for DREADD mutations

Promoter

human Synapsin 1

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pEGFP-parkin C431S

Plasmid

#45877

Depositor

Edward Fon

Publication

Trempe et al Science. 2013 May 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

C431S mutation (ubiquitin acceptor site)

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Publication

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Tags

6His

Expression

Bacterial

Promoter

T5

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M-ST1cas

Plasmid

#48669

Purpose

Mammalian S. thermophilus #1 Cas9 expression, human optimized

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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EGFP-hCaM

Plasmid

#47602

Depositor

Emanuel Strehler

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CaM (CALM3 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP-C1-FLAG-XRCC4

Plasmid

#46959

Purpose

Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Publication

Britton et al J Cell Biol. 2013 Jul 29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

XRCC4 (XRCC4 Human)

Tags

GFP-2xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Publication

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p75-RFP

Plasmid

#24092

Depositor

Moses Chao

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p75NTR (NGFR Human)

Tags

DSRed

Expression

Mammalian

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-AKAR2-CR

Plasmid

#40255

Depositor

Michael Lin

Publication

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AKAR2

Tags

His6 and Xpress

Expression

Mammalian

Promoter

CMV IE

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

Plasmid

#39761

Depositor

Pamela Raymond

Publication

Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-1

Insert

zebrafish gfap promoter- EGFP (gfap Zebrafish)

Use

Zebrafish expression

Promoter

gfap

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3

Plasmid

#32739

Depositor

Frank McCormick

Publication

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TCF4E (Tcf7l2 Human)

Tags

Myc

Expression

Mammalian

Mutation

The N-terminal deletion form (delta N) lacks amin…

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRE-Slow-FT

Plasmid

#31915

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Slow-FT

Expression

Mammalian

Mutation

M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

Promoter

PTight Tet-responsive PminCMVd

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-F3

Plasmid

#32634

Depositor

Edward Callaway

Publication

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

B19N, B19P, B19M and B19L

Expression

Mammalian

Mutation

In frame insertion of Ser after aa10 in M (matrix…

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pRS3 (CK2alpha, CK2beta)

Plasmid

#27092

Depositor

David Litchfield

Publication

Turowec et al Methods Enzymol. 2010;484:471-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CK2beta (CSNK2B Human)
CK2alpha (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA and Myc

Expression

Mammalian

Promoter

bidirectional tet-responsive promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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NGFR

Plasmid

#27489

Depositor

Warren Pear

Publication

Izon et al Immunity. 2001 Mar . 14(3):253-64.

Type

Empty backbone

Use

Retroviral

Tags

ECMV, IRES, and NGFR

Expression

Mammalian

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLenti CMVtight Neo DEST (w770-1)

Plasmid

#26432

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Expression

Mammalian

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CMV::ratSyGCaMP2

Plasmid

#26124

Purpose

Expression of SyGCaMP2 (rat synaptophysin fused to GCaMP2) with CMV promoter

Depositor

Leon Lagnado

Publication

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Synaptophysin GCaMP2 (Syp Rat)

Expression

Mammalian

Available since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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