165,775 results

1. pRS314-T7-GFP

   Plasmid

   #33130

   Depositor

   Michael Rosbash

   Article

   Dower et al RNA. 2002 May;8(5):686-97.

   Insert

   GFP with TDH3 5' and 3' UTR (gfp )
   

   Expression

   Yeast

   Promoter

   T7

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC3109 (pSpy1C-Plac(1)_ffluc)

   Plasmid

   #218514

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of mrfp cassette, mutagenesis of the RBS…

   Promoter

   Plac(1)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOpen-NotIR (GC)

   Plasmid

   #165507

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   NotI (GC-rich recognition site)
   

   Use

   Synthetic Biology

   Available Since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSFFV_sfCherry3C(1-10)

   Plasmid

   #117482

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3C(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3C(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   5 substitutions (K45R, G52D, T106A, K182R, N194D)…

   Promoter

   SFFV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-CMV-GFP-W

   Plasmid

   #196046

   Purpose

   lentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoter

   Depositor

   Darrell Kotton

   Article

   Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   eGFP

   Promoter

   CMV

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-pcDNA3.1 Puro-CAG-Positron2

   Plasmid

   #239079

   Purpose

   Mammalian expression of positive-going voltage sensor

   Depositor

   GENIE Project

   Article

   Eric R. et al Janelia Figshare 2023

   Insert

   Positron2
   

   Expression

   Mammalian

   Mutation

   R78K N81D D92N W178F

   Promoter

   CAG

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH2B-miRFP680-N1

   Plasmid

   #197229

   Purpose

   Expresses the fusion protein of H2B-miRFP680 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   H2B-miRFP680
   

   Expression

   Mammalian

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. hGFAP-GFP

   Plasmid

   #40592

   Depositor

   Albee Messing

   Article

   Zhuo et al Dev Biol. 1997 Jul 1;187(1):36-42.

   Insert

   GFP-S65T
   

   Use

   Mouse Targeting

   Tags

   MP-1 fragment (mouse protamine 1 intron and polya…

   Expression

   Bacterial and Mammalian

   Mutation

   Construct contains a mutated, humanized hGFP(S65T…

   Promoter

   hGFAP promoter

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag p21 WT

   Plasmid

   #16240

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

   Insert

   p21 (CDKN1A Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104910

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

    Plasmid

    #178960

    Purpose

    Expresses saCas9 protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Staphylococcus aureus Cas9 (SaCas9)
    

    Use

    AAV

    Tags

    3x HA

    Promoter

    gfaABC1D (astrocyte specific)

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. NES-Grx1-roGFP2

    Plasmid

    #236551

    Purpose

    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    NES-Grx1-roGFP2 (NES Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLysscys255

    Plasmid

    #36429

    Depositor

    Emine Gunhan, Pete Lollar

    Article

    Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

    Insert

    Saporin-3/cys255
    

    Expression

    Bacterial

    Mutation

    cys added at position 255

    Promoter

    T7

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_gRNA -hMLHdn-Puro

    Plasmid

    #191105

    Purpose

    Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    I31 silent mutation (ATA to ATC) to remove early …

    Promoter

    EF-1a

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUNIV backbone

    Plasmid

    #24705

    Depositor

    Cynthia Czajkowski

    Article

    Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

    Type

    Empty backbone

    Use

    Xenopus oocyte expression

    Expression

    Bacterial, Mammalian, and Y…

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40

    Plasmid

    #179120

    Purpose

    Expresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    control sgRNA
    

    Use

    AAV

    Promoter

    U6

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GPRC6A-Tango

    Plasmid

    #66386

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPRC6A (GPRC6A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid

    #20298

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

    Plasmid

    #138610

    Purpose

    Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Inserts

    Hyperactive AID
    T7 RNA Polymerase
    tdTomato
    

    Expression

    Mammalian

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAct:dCas9-VPR

    Plasmid

    #78898

    Purpose

    Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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