p1242 3x-KB-L
(Plasmid #26699)

• Depositing Lab: Bill Sugden
• Publication: Mitchell et al J Virol. 1995 May . 69(5):2968-76.

Backbone

• Vector backbone: pGL2-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3x MHC I KB element - luciferase
• Alt name: MHC I NFKB element
• Alt name: luciferase
• Species: H. sapiens (human); Photinus pyralis
• Insert Size (bp): 200
• Entrez Gene: NFKB1 (a.k.a. CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (destroyed during cloning)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: EBV Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Baldwin, A. S., Jr., J. C. Azizkhan, D. E. Jensen, A. A. Beg, and L. R. Coodly. 1991. Induction of NF-KB DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts. Mol. Cell. Biol. 11:4943–4951.
• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid 3X-KB-L has a minimal fos promoter element and three copies of the major histocompatibility complex (MHC) class I KB element TGGGGATTCCCCA. It was constructed by ligation of the fragment obtained through digestion of MHC-NF-KB CAT by HindIII, blunting with Klenow DNA polymerase, and then digestion with BamHI. The fragment was ligated to pGL2-Basic digest(SmaI+BglII).