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pGBMCS-SortA
(Plasmid #21931)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21931 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21d
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL-21(DE3) and its derivatives can be used for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sortase
  • Alt name
    srtA
  • Alt name
    sortA
  • Alt name
    sortase A
  • Species
    Staphylococcus aureus (strain N315)
  • Insert Size (bp)
    627
  • Mutation
    deleted amino acids 1-59
  • GenBank ID
    SA2316
  • Entrez Gene
    srtA
  • Tags / Fusion Proteins
    • GB1 (N terminal on insert)
    • His-tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We obtained srtA gene from NBRC and cloned it into expression vector.
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBMCS-SortA was a gift from Fuyuhiko Inagaki (Addgene plasmid # 21931 ; http://n2t.net/addgene:21931 ; RRID:Addgene_21931)
  • For your References section:

    Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method. Kobashigawa Y, Kumeta H, Ogura K, Inagaki F. J Biomol NMR. 2009 Mar . 43(3):145-50. 10.1007/s10858-008-9296-5 PubMed 19140010