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pDEST51-LRRK2-WT
(Plasmid #25080)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25080 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 25080-D50 50 μg of DNA in Tris buffer $495
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DNA 25080-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEF-DEST51
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7464
  • Vector type
    Mammalian Expression, Gateway Cloning
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LRRK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7584
  • Entrez Gene
    LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • 6XHis (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST51-LRRK2-WT was a gift from Mark Cookson (Addgene plasmid # 25080 ; http://n2t.net/addgene:25080 ; RRID:Addgene_25080)

  • For your References section:

    The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation. . J Biol Chem. 2008 Jun 13;283(24):16906-14. 10.1074/jbc.M708718200 PubMed 18397888

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