|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16591||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePUMA promotor fragment
SpeciesH. sapiens (human)
Insert Size (bp)493
Entrez GeneBBC3 (a.k.a. JFY-1, JFY1, PUMA)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Contains consensus p53 binding sites BS1 and BS2, lying 230 and 144 bp upstream of the transcription start site. 5' end is 336 bp upstream of the putative transcription start site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PUMA Frag1-Luc was a gift from Bert Vogelstein (Addgene plasmid # 16591 ; http://n2t.net/addgene:16591 ; RRID:Addgene_16591)
For your References section:PUMA induces the rapid apoptosis of colorectal cancer cells. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. Mol Cell. 2001 Mar . 7(3):673-82. 10.1016/S1097-2765(01)00213-1 PubMed 11463391
Map uploaded by the depositor.