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pBS human c-Myc

Plasmid

#14971

Depositor

Joan Massague

Publication

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (MYC Human)

Expression

Bacterial

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Hmga2 3'UTR wt luciferase (Luc-wt)

Plasmid

#14785

Depositor

David Bartel

Publication

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Luc-wt (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEP PolI

Plasmid

#11722

Depositor

Lawrence Loeb

Publication

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I EP (polA E. coli)

Expression

Bacterial

Mutation

D424A, I709N, A759R. Error-prone version of PolI.

Available since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFLAG/HA-MOV10

Plasmid

#10976

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MOV10 (MOV10 Human)

Tags

FLAG and HA

Expression

Mammalian

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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EGFR G719S

Plasmid

#11013

Purpose

Retroviral construct for expressing human EGFR with G719S mutation in human cells

Depositor

Matthew Meyerson

Publication

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR G719S (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

G719S

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-evoCAST-eCas8

Plasmid

#234727

Purpose

Engineered human-codon optimized PseCas8 in mammalian cells (for evoCAST)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ePseCas8

Expression

Mammalian

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-evoCAST-TniQ

Plasmid

#234728

Purpose

Engineered human-codon optimized PseTniQ in mammalian cells (for evoCAST)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PseTniQ

Expression

Mammalian

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9

Plasmid

#182310

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA-T2A-SOX9 (NFIA, SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1

Plasmid

#171614

Purpose

AsCas12f1-based Human cell genome editing

Depositor

Quanjiang Ji

Publication

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AsCas12f1 and sgRNA_v1

Expression

Mammalian

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p-EF1a-CreERT2-3Xflag-T2A-eBFP2

Plasmid

#170186

Purpose

This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

Depositor

Bradley Bernstein

Publication

Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

Insert

CreERT2-T2A-eBFP2

Use

Lentiviral

Promoter

EF1 alpha

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 TFAM-mScarlet

Plasmid

#129573

Purpose

Expression of mScarlet fused TFAM

Depositor

Stephen Tait

Publication

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAR-Ec633

Plasmid

#130873

Purpose

A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

Depositor

Chang Liu

Publication

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Mutation

L477V, L640Y, I777K, W814N

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Publication

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMIH-TFAM-GFP

Plasmid

#113704

Purpose

Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

Depositor

Benjamin Kile

Publication

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TFAM (Tfam Mouse)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Promoter

MSCV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMXs GFP-LC3-RFP

Plasmid

#117413

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

Depositor

Noboru Mizushima

Publication

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR-Tre3G-29xGGGGCC-12xMS2

Plasmid

#99149

Purpose

Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.

Depositor

Ron Vale

Publication

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

29xGGGGCC 12xMS2 hairpins

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLPCX2-MT1-MMP-eGFP

Plasmid

#89819

Purpose

Express in MT1-MMP mammalian cells

Depositor

Shengyu Yang

Publication

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

MT1-MMP (MMP14 Human)

Use

Retroviral

Tags

eGFP

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-Nanoluc-ccdB

Plasmid

#87078

Purpose

Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Use

Lentiviral

Tags

Nanoluc luciferase

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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