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Programmed genome editing by a miniature CRISPR-Cas12f nuclease.
Wu Z, Zhang Y, Yu H, Pan D, Wang Y, Wang Y, Li F, Liu C, Nan H, Chen W, Ji Q
Nat Chem Biol. 2021 Sep 2. pii: 10.1038/s41589-021-00868-6. doi: 10.1038/s41589-021-00868-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
171609p15a-AsCas12f-blaZAsCas12f1-based E. coli genome editing
171610p15a-AsCas12f-apmRAsCas12f1-based K. pneumoniae genome editing
171611psgRNAv1-emptysgRNA_v1 plasmid for AsCas12f1 in bacteria
171612pET28a-SUMO-AsCas12fAsCas12f1 heterologous expression plasmid
171613pET28a-SUMO-AsCas12f-2NLSNLS-AsCas12f1-NLS heterologous expression plasmid for RNP
171614pCMV-AsCas12f1AsCas12f1-based Human cell genome editing

Antibodies from Article