Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pEP PolI
(Plasmid #11722)


Item Catalog # Description Quantity Price (USD)
Plasmid 11722 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    see NCBI D88215
  • Backbone size w/o insert (bp) 3475
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Grow at 30oC in LB in JS200 cells (polA recA718). The cells need to be kept in exponential phase (do not let the culture reach saturation)!
  • Copy number
    Low Copy


  • Gene/Insert name
    DNA polymerase I EP
  • Alt name
    DNA polymerase I
  • Alt name
  • Alt name
  • Species
    E. coli
  • Insert Size (bp)
  • Mutation
    D424A, I709N, A759R. Error-prone version of PolI.
  • Entrez Gene
    polA (a.k.a. b3863, ECK3855, JW3835, resA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer m13 reverse primer
  • 3′ sequencing primer m13 forward 20 primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See attached mutagenesis protocol provided by depositing scientist.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEP PolI was a gift from Lawrence Loeb (Addgene plasmid # 11722 ; ; RRID:Addgene_11722)
  • For your References section:

    Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I. Camps M, Naukkarinen J, Johnson BP, Loeb LA. Proc Natl Acad Sci U S A. 2003 Aug 19. 100(17):9727-32. 10.1073/pnas.1333928100 PubMed 12909725