Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #11724)


Item Catalog # Description Quantity Price (USD)
Plasmid 11724 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    modified pGPS3
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 2642
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
    ampicillin resistance
  • Alt name
    carbenicillin resistance
  • Species
    E. coli
  • Insert Size (bp)
  • Mutation
    GAA for Glu26 changed to TAA (premature stop codon introduced in beta-lactamase gene).

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AflIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance.

The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI.

Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLA230 was a gift from Lawrence Loeb (Addgene plasmid # 11724 ; ; RRID:Addgene_11724)
  • For your References section:

    In vivo mutagenesis by Escherichia coli DNA polymerase I. Ile(709) in motif A functions in base selection. Shinkai A, Loeb LA. J Biol Chem. 2001 Dec 14. 276(50):46759-64. 10.1074/jbc.M104780200 PubMed 11602576