163,354 results

1. pcDNA3.1 SARS-CoV-2 N

   Plasmid

   #158079

   Purpose

   Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 nucleocapsid (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEX-SaCas9-U6-sgRNA

   Plasmid

   #124844

   Purpose

   Vector for Cre-dependent expression of SaCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SaCas9, gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS and NLS-3xHA

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-SLC38A10_STOP

   Plasmid

   #161249

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC38A10 (SLC38A10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLEX-MCS-ASC-GFP

   Plasmid

   #73957

   Purpose

   Express human ASC with a C-terminal EGFP tag in mammalian cells

   Depositor

   Christian Stehlik

   Article

   de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

   Insert

   PYD and CARD domain containing, PYCARD (PYCARD Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY109 (lenti-LbCpf1)

   Plasmid

   #84740

   Purpose

   Lenti virus delivery of LbCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   Lentiviral

   Tags

   3xHA, NLS, and P2A

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM4Z-Fluc

   Plasmid

   #183474

   Purpose

   This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

   Depositor

   Chantal Pichon

   Article

   Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINB

   Plasmid

   #41142

   Depositor

   Ray Owens

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLX-PGK-hTERT

   Plasmid

   #114315

   Purpose

   Constitutive expression of the hTert protein

   Depositor

   Patrick Salmon

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PCR4-Shh::lacZ-H11

   Plasmid

   #139098

   Purpose

   enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

   Depositor

   Len Pennacchio

   Article

   Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Shh

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRESCA-syntide

    Plasmid

    #132415

    Purpose

    FRET-based sensor for CaMKII activity

    Depositor

    Margaret Stratton

    Article

    Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

    Insert

    FRESCA-syntide
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

    Plasmid

    #198035

    Purpose

    Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    EGFP and GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. BII-ChPtW-iRFP670

    Plasmid

    #133397

    Purpose

    Piggybac vector for constitutive expression of iRFP670-NLS

    Depositor

    Blair Madison

    Insert

    iRFP-670-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNIC28-SENP2

    Plasmid

    #196355

    Purpose

    protein expression of the His-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP2 catalytic domain
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    contains only D364-L589

    Promoter

    T7

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ-CBE-Cas9n-Act3.0

    Plasmid

    #178955

    Purpose

    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX307-CTSB-blast

    Plasmid

    #158464

    Purpose

    Constitutive lentiviral expression of human CTSB.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    CTSB (CTSB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-enCas12a-A3A-Y130F

    Plasmid

    #193652

    Purpose

    Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-hA3A-Y130F, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENN239_CRISPRcharm_Kv1

    Plasmid

    #220838

    Purpose

    CRISPRcharm Kv1 expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm Kv1
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-dCas12a-A3A-Y132D

    Plasmid

    #193641

    Purpose

    Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3A-Y132D
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A-Y132D

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3HA-ERHBD-Hoxb8

    Plasmid

    #212932

    Purpose

    Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

    Depositor

    Hans Haecker

    Article

    Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

    Insert

    3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Triple HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21-pelB-LaM-6

    Plasmid

    #172771

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-6
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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