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HA-FOXO3a TM-ER
(Plasmid #8353)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8353 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FOXO3a
  • Alt name
    FKHRL1
  • Alt name
    forkhead family
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Mutation
    T32A, S253A, S315A, mouse estrogen receptor (G525R) on C-term
  • GenBank ID
    NP_001446 NP_031982
  • Entrez Gene
    FOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
  • Tag / Fusion Protein
    • HA (YDVPDYASL) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Mutations in phosphorylation sites. Has ER--ligand binding domain of estrogen receptor. See wild-type map and sequence under HA-FOXO3a WT-ER.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-FOXO3a TM-ER was a gift from Michael Greenberg (Addgene plasmid # 8353)
  • For your References section:

    DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein. Tran H, Brunet A, Grenier JM, Datta SR, Fornace AJ Jr, DiStefano PS, Chiang LW, Greenberg ME. Science 2002 Apr 19;296(5567):530-4. 10.1126/science.1068712 PubMed 11964479