|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8353||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2900
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameforkhead family
SpeciesH. sapiens (human)
Insert Size (bp)3000
MutationT32A, S253A, S315A, mouse estrogen receptor (G525R) on C-term
GenBank IDNP_001446 NP_031982
Entrez GeneFOXO3 (a.k.a. AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
/ Fusion Protein
- HA (YDVPDYASL) (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SV40pro-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Mutations in phosphorylation sites. Has ER--ligand binding domain of estrogen receptor. See wild-type map and sequence under HA-FOXO3a WT-ER.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HA-FOXO3a TM-ER was a gift from Michael Greenberg (Addgene plasmid # 8353)
For your References section:DNA repair pathway stimulated by the forkhead transcription factor FOXO3a through the Gadd45 protein. Tran H, Brunet A, Grenier JM, Datta SR, Fornace AJ Jr, DiStefano PS, Chiang LW, Greenberg ME. Science 2002 Apr 19;296(5567):530-4. 10.1126/science.1068712 PubMed 11964479