PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs.
Foran G, Hallam RD, Megaly M, Turgambayeva A, Necakov A
Biotechniques. 2023 Oct;75(4):168-178. doi: 10.2144/btn-2023-0042. Epub 2023 Oct 10. PubMed Article
Foran G, Hallam RD, Megaly M, Turgambayeva A, Necakov A
Biotechniques. 2023 Oct;75(4):168-178. doi: 10.2144/btn-2023-0042. Epub 2023 Oct 10. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203297 | PlayBack-U6-Kpn1 | For expression of sgRNAs in a PlayBack Vector with Kpn1 as a PlayBack Restriction Enzyme |
| 203298 | PlayBack-U6-Bamh1 | For expression of sgRNAs in a PlayBack Vector with Bamh1 as a PlayBack Restriction Enzyme |
| 203299 | PlayBack-U6-Bgl11 | For expression of sgRNAs in a PlayBack Vector with BglII as a PlayBack Restriction Enzyme |
| 203300 | PlayBack-U6-Xba1 | For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme |
| 203301 | PlayBack-U6-Not1 | For expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction Enzyme |
| 203302 | PlayBack-CMV-Kpn1 | For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme |
| 203303 | PlayBack-CMV-Bamh1 | For expression of eukaryotic genes of interest with CMV promoter with Bamh1 as a PlayBack Restriction Enzyme |
| 203304 | PlayBack-CMV-Nhe1 | For expression of eukaryotic genes of interest with CMV promoter with NheI as a PlayBack Restriction Enzyme |
| 203305 | PlayBack-CMV-EcoR1 | For expression of eukaryotic genes of interest with CMV promoter with EcoRI as a PlayBack Restriction Enzyme |
| 203306 | PlayBack-CMV-Bgl11 | For expression of eukaryotic genes of interest with CMV promoter with BglII as a PlayBack Restriction Enzyme |
| 203307 | PlayBack-Ef1aKpn1 | For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme |
| 203308 | PlayBack-Ef1a-Bamh1 | For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme |
| 203309 | PlayBack-Ef1a-Nde1 | For expression of eukaryotic genes of interest with Ef1a promoter with NdeI as a PlayBack Restriction Enzyme |
| 203310 | PlayBack-Ef1a-Nhe1 | For expression of eukaryotic genes of interest with Ef1a promoter with NheI as a PlayBack Restriction Enzyme |
| 203311 | PlayBack-Ef1a-EcoR1 | For expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction Enzyme |
| 203312 | PiggyBac PlayBack | To all for PiggyBac gene insertion into Genome |