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pORI28
(Plasmid #71595)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71595 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pWV01
  • Backbone size (bp) 2200
  • Modifications to backbone
    RepA-
  • Vector type
    integration vector for bacteria

Growth in Bacteria

  • Bacterial Resistance(s)
    Erythromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli EC1000 provides RepA in trans
  • Growth instructions
    E. coli EC1000 required as it provides RepA in trans. This strain needs to be grown in 150 micrograms/mL erythromycin for pORI28 replication as well as 40 micrograms per mL of kanamycin to maintain repA in EC1000 chromosome). Please note that EC1000 (#71852) is available from Addgene and can be ordered at the same time for use as the cloning host of pORI28.
  • Copy number
    Low Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was created by Jan Kok et al., Department of Genetics, Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 BB Haren, The Netherlands
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pORI28 was a gift from Todd Klaenhammer & Jan Kok (Addgene plasmid # 71595 ; http://n2t.net/addgene:71595 ; RRID:Addgene_71595)
  • For your References section:

    A general system for generating unlabelled gene replacements in bacterial chromosomes. Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I, Dabrowska M, Venema G, Kok J. Mol Gen Genet. 1996 Nov 27;253(1-2):217-24. PubMed 9003306