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pTRKH2
(Plasmid #71312)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71312 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIL253
  • Backbone size (bp) 4963
  • Modifications to backbone
    insertion pSA34, pIC-19R MCS
  • Vector type
    Bacterial Expression ; E. coli-gram positive bacteria shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Erythromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM110
  • Growth instructions
    Erythromycin selection is 150 ug/ml in E. coli
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRKH2 was a gift from Rodolphe Barrangou & Todd Klaenhammer (Addgene plasmid # 71312 ; http://n2t.net/addgene:71312 ; RRID:Addgene_71312)
  • For your References section:

    High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening. O'Sullivan DJ, Klaenhammer TR. Gene. 1993 Dec 31;137(2):227-31. PubMed 8299952