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Depositing Lab
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Depositing OrganizationScripps California (The Scripps Research Institute)
Located in the United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12438 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 12438-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 12438-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 4790
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUBE1L
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SpeciesH. sapiens (human)
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Entrez GeneUBA7 (a.k.a. D8, UBA1B, UBE1L, UBE2, UBE7)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI? (not destroyed)
- 5′ sequencing primer pCAG-F
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byUBE1L cDNA was provided by Charles Buys and subcloned into pCAGGS
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains a R87G mutation in UBE1L. It is not known how this mutation impacts plasmid function.
DNA (Catalog # 12438-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 12438-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-HA-hUBE1L was a gift from Dong-Er Zhang (Addgene plasmid # 12438 ; http://n2t.net/addgene:12438 ; RRID:Addgene_12438) -
For your References section:
Interferon-inducible ubiquitin E2, Ubc8, is a conjugating enzyme for protein ISGylation. Kim KI, Giannakopoulos NV, Virgin HW, Zhang DE. Mol Cell Biol. 2004 Nov . 24(21):9592-600. 10.1128/MCB.24.21.9592-9600.2004 PubMed 15485925
