161,273 results

1. plenti6-SLC7A11/xCT-V5

   Plasmid

   #170427

   Purpose

   Eepresses SLC7A11 (also named xCT) in mammalian cells

   Depositor

   Boyi Gan

   Article

   Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

   Insert

   solute carrier family 7 member 11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

   Plasmid

   #183411

   Purpose

   piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   Tet-on 3G
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCag SE (Self-excising) FlpO-2A-Cre EV

   Plasmid

   #130986

   Purpose

   episomal expression of FlpO and Cre recombinases, self excising

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   protamine intron added to FlpO to prevent bacteri…

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18-GFP

   Plasmid

   #220495

   Purpose

   To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Use

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

   Plasmid

   #199604

   Purpose

   Expression of cell permeable spCas9 in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   Cas9-T6N
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2058

   Plasmid

   #141898

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX2-2 (NKX2-2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEGFP-HRas

   Plasmid

   #18662

   Depositor

   Karel Svoboda

   Article

   Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-3xFLAG-Blast

   Plasmid

   #207717

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCAG-Fab_8D3_2_H-H6

    Plasmid

    #176075

    Purpose

    Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    His6 tagged heavy chain of Fab_8D3_2
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Fab_8D3_2_L

    Plasmid

    #176076

    Purpose

    Express light chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    Light chain of Fab_8D3_2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV14_Fibrillarin

    Plasmid

    #73061

    Purpose

    expression clone for human Fibrillarin with C-terminal 3xFLAG-tag

    Depositor

    Stefan Stamm

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209729

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3365

    Plasmid

    #144841

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCB306

    Plasmid

    #89360

    Purpose

    sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ALIX-mNeonGreen

    Plasmid

    #191191

    Purpose

    Full-length ALIX with C-terminal mNeonGreen tag.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (PDCD6IP Synthetic, Human)
    

    Use

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.Syn.GCaMP6f.WPRE.SV40

    Plasmid

    #100837

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Cre-IRES2-GFP

    Plasmid

    #26646

    Purpose

    Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

    Depositor

    Anjen Chenn

    Article

    Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    GFP and IRES2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL11017 (pICH47732::NOSp-BAR-NOST)

    Plasmid

    #51145

    Purpose

    Confers Basta resistance in planta

    Depositor

    Jonathan D Jones

    Insert

    NOSp-BAR-NOST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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