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pMP71-HLA-B0702-His

Plasmid

#104850

Purpose

HLA-B0702 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-B Human)

Use

Retroviral

Tags

his

Expression

Mammalian

Promoter

LTR

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-G-NG

Plasmid

#220897

Purpose

Express haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Tags

Citrine

Expression

Mammalian

Promoter

3xMnx1

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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GPR149-Tango

Plasmid

#66324

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR149 (GPR149 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

Plasmid

#123614

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF64-2xNSTREP

Plasmid

#136224

Purpose

Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAdEasy-1

Plasmid

#16400

Purpose

Adenoviral vector for use in AdEasy System.

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a (JDW 1164)

Plasmid

#224473

Purpose

Gateway compatible 5' entry clone containing the human EF1a promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF1a promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a-TN

Plasmid

#86077

Purpose

A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed threonine 22 to aspargine

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEN396-pCAGGS-TIR1-V5-2A-HygroR

Plasmid

#195102

Purpose

Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

TIR1

Tags

V5

Expression

Mammalian

Promoter

pCAGGS

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-Nucleocapsid

Plasmid

#168886

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-Nucleocapsid (N )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR18Q

Plasmid

#87228

Purpose

HTT HR arms with 18 CAG repeats and piggyBac selection cassette

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm

Expression

Mammalian

Promoter

EF1a driving selection cassette

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHCT2

Plasmid

#130970

Purpose

HyPr plasmid with natMX marker

Depositor

Chris Todd Hittinger

Article

Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

Insert

empty backbone

Expression

Yeast

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pIVTR(T7A)-E3

Plasmid

#172305

Purpose

In vitro transcription of E3

Depositor

Patrik Verstreken

Insert

E3

Use

Other

Promoter

T7 promoter A-initiating

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCK301

Plasmid

#87767

Purpose

E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

Insert

PrhaBAD-sfGFP

Use

Synthetic Biology

Tags

6xHis Tag

Expression

Bacterial

Promoter

PrhaBAD rhamnose-inducible promoter from E. coli

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLacO-ISce1

Plasmid

#58505

Purpose

provides 256 Lac operator repeats adjacent to the ISce1 restriction site

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Lac repeats adjacent to 6 copies of the ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-MITF

Plasmid

#64888

Purpose

Retroviral expression of murine MITF

Depositor

Ivana de la Serna

Article

de la Serna et al J Biol Chem. 2006 Jul 21;281(29):20233-4

Insert

MITF (Mitf Mouse)

Use

Retroviral

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pSLIK-Neo

Plasmid

#25735

Purpose

3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-HygroR-T2A-TetOn3G

Plasmid

#179887

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

Hygromycin resistance

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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