pHage2-EF1a-TERT-IRES-Puro
(Plasmid #182590)

• Purpose: expresses TERT in mammalian cells
• Depositing Lab: Agnel Sfeir
• Publication: Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. doi: 10.1016/j.molcel.2020.05.005. Epub 2020 Jun 3.

Backbone

• Vector backbone: pHAGE2
• Backbone size w/o insert (bp): 8300
• Total vector size (bp): 11633
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hTERT
• Species: H. sapiens (human)
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
• Promoter: EF-1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: ttcttccatttcaggtgtc
• 3′ sequencing primer: AGGAATGCTCGTCAAG

Resource Information

• Supplemental Documents:
  • 1407-pHAGE2-EF1a-hTERT-IRES-puro (1).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry