165,537 results

1. EGFP-mCherry

   Plasmid

   #193553

   Purpose

   Expresses mEGFP-mCherry in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   mCherry
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGADT7-GW

   Plasmid

   #61702

   Purpose

   Gateway compatible Y2H vector - AD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_GPHN.FingR-eGFP-CCR5TC

   Plasmid

   #46296

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR-eGFP-CCR5TC (GPHN Human, Synthetic)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP scFv [N86/44]

   Plasmid

   #206743

   Purpose

   Mammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   GFP (Aequorea victoria) recombinant scFV
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Lyso-TORCAR

   Plasmid

   #64929

   Purpose

   Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   LAMP1-TORCAR (LAMP1 Human, Synthetic)
   

   Tags

   Cerulean, Full-length LAMP1, and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-BRX-eGFP-NLS

   Plasmid

   #217534

   Purpose

   AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   BMP reporter element and miniXon casette (Smad1 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   BMP Reporter Element

   Available Since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMV GFP DEST (736-1)

   Plasmid

   #19732

   Purpose

   3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Gateway destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF CREB

   Plasmid

   #22968

   Depositor

   Marc Montminy

   Article

   Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

   Insert

   CREB (Creb1 Rat)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH41766

   Plasmid

   #48018

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid

    #215014

    Purpose

    Express an autophagy (LC3) reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    MAP1LC3B (Map1lc3b Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Blast-dTAG

    Plasmid

    #207683

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pKrox24(2xD-E_inD)Luc

    Plasmid

    #200109

    Purpose

    Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element in front of EGR1 promoter…

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLminP_Luc2P_RE6

    Plasmid

    #90340

    Purpose

    STAT5 - Stat5 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    STAT5

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eDrIR1

    Plasmid

    #183998

    Purpose

    Optically controlled eDrIR1 expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrIR1 (INSR Human)
    

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. prhaBAD-pA-Hia5

    Plasmid

    #222303

    Purpose

    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    pA-Hia5
    

    Tags

    6His and protein A

    Expression

    Bacterial

    Mutation

    codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-hHSF1 WT

    Plasmid

    #71724

    Purpose

    plasmid expressing myc-his tag hHSF1 WT

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    HSF1 (HSF1 Human)
    

    Tags

    MYC 6xHIS

    Expression

    Mammalian

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5154

    Plasmid

    #175390

    Purpose

    High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)

    Depositor

    Michael Wiles

    Article

    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting; Cloning vector, to be used to de…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-PML IV/pRK5

    Plasmid

    #59742

    Purpose

    Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

    Depositor

    Xiaolu Yang

    Article

    Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

    Insert

    PML isoform IV (PML Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET23b-NTF2

    Plasmid

    #108918

    Purpose

    Expresses NTF2

    Depositor

    Larry Gerace

    Article

    Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

    Insert

    NTF2
    

    Expression

    Bacterial

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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