159,237 results

1. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ

   Plasmid

   #196224

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLNCX2-CFP-VGPDFGR-YFP

   Plasmid

   #59587

   Purpose

   Encodes a granzyme B FRET reporter

   Depositor

   Tim Mitchison

   Article

   Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

   Insert

   ECFP-VGPDFGR-Venus
   

   Use

   Retroviral

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPR-EGFP

   Plasmid

   #75159

   Purpose

   Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.3-hALDOA

   Plasmid

   #210809

   Purpose

   For transient transfection

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PL-SIN-EOS-S(4+)-EGFP

   Plasmid

   #21317

   Purpose

   Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EOS-S(4+) promoter region

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-RUNX2

   Plasmid

   #192926

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   RUNX2 (RUNX2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPRT::DHFR-D

   Plasmid

   #58528

   Purpose

   replace TgUPRT with DHFR by homologous recombination

   Depositor

   David Sibley

   Article

   Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

   Insert

   DHFR cassette
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   pyrimethamine resistant

   Promoter

   DHFR

   Available since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX208 CMV sTurboID (C)-HA-FRB-ERM

   Plasmid

   #153007

   Purpose

   Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)-ERM (Cb5)
   

   Use

   Lentiviral

   Tags

   FRB and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pet28a_Human_H4

   Plasmid

   #42633

   Depositor

   Joe Landry

   Insert

   Human H4 (H4C1 Human)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pNP1830

    Plasmid

    #222728

    Purpose

    pNP561 - T5 Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKEN GFP mut2

    Plasmid

    #20409

    Depositor

    Raphael Valdivia, Stanley Falkow

    Article

    Cormack et al Gene. 1996 . 173(1 Spec No):33-8.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S65A V68L S72A

    Promoter

    Available since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-TRE-DIO-eGFP-2A-TetanusToxin

    Plasmid

    #166603

    Purpose

    Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    EGFP-2A-Tetanus Toxin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AICSDP-47: CTNNB1-mEGFP

    Plasmid

    #109119

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1

    Depositor

    Allen Institute for Cell Science

    Insert

    CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. lentiTet-P2A-mCherry BlastR

    Plasmid

    #186743

    Purpose

    Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    P2A-mcherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLminP_Luc2P_RE4

    Plasmid

    #90338

    Purpose

    NRF2 - Antioxidant response element gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NRF2

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Anti-6xHis [N144/14R]

    Plasmid

    #177500

    Purpose

    Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-6xHis recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30(a)-AtNUDX23

    Plasmid

    #175021

    Purpose

    Purification of AtNUDX23 enzyme

    Depositor

    Jeppe Vinther

    Article

    Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

    Insert

    AtNUDX23 (NUDX23 Mustard Weed)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pES001 MBP-GSK3β-HA-His

    Plasmid

    #154010

    Purpose

    Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Tags

    HA tag, His6, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pREDCas9

    Plasmid

    #71541

    Purpose

    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    lambda red genes
    plasmid curing system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pemiRFP703-N1

    Plasmid

    #136558

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP703
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge