|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19763||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9941
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1320
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
/ Fusion Protein
- 3xHA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV-tetO-myc T58A was a gift from Konrad Hochedlinger (Addgene plasmid # 19763 ; http://n2t.net/addgene:19763 ; RRID:Addgene_19763)
For your References section:Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Stadtfeld M, Maherali N, Breault DT, Hochedlinger K. Cell Stem Cell. 2008 Mar 6. 2(3):230-40. 10.1016/j.stem.2008.02.001 PubMed 18371448