|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19766||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9941
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1100
Entrez GenePou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
Note that the region immediately upstream of the insert in the author's sequence is not 100% accurate. See Addgene's quality control sequence for this region.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV-tetO-Oct4 was a gift from Konrad Hochedlinger (Addgene plasmid # 19766 ; http://n2t.net/addgene:19766 ; RRID:Addgene_19766)
For your References section:Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Stadtfeld M, Maherali N, Breault DT, Hochedlinger K. Cell Stem Cell. 2008 Mar 6. 2(3):230-40. 10.1016/j.stem.2008.02.001 PubMed 18371448