pcDNA 3.1(-) mouse C/EBP delta
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12559||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5427
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameCCAAT/Enhancer-binding Protein delta
Alt nameC/EBP delta
SpeciesM. musculus (mouse)
Insert Size (bp)807
MutationS2G introduced during cloning
Entrez GeneCebpd (a.k.a. c/EBPdelta)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
When this C/EBPdelta construct was created, an NcoI restriction site was introduced that overlaps the start (AUG) codon to produce an optimal translation initiation site. This cloning strategy resulted in a S2G mutation in C/EBPdelta as a cloning "scar."
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA 3.1(-) mouse C/EBP delta was a gift from Peter Johnson (Addgene plasmid # 12559 ; http://n2t.net/addgene:12559 ; RRID:Addgene_12559)