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pcDNA flag PPAR gamma E499Q
(Plasmid #8896)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8896 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 8896-D50 50 μg of DNA in Tris buffer $495
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DNA 8896-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1 myc-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAR gamma
  • Alt name
    PPARg
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1547
  • Mutation
    E499Q, a mutant in the AF2 domain. Binds PPARg ligands with comparable affininty as wild-type, but no ligand-dependent transcriptional activation.
  • GenBank ID
    NM_011146
  • Entrez Gene
    Pparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA flag PPAR gamma E499Q was a gift from Bruce Spiegelman (Addgene plasmid # 8896 ; http://n2t.net/addgene:8896 ; RRID:Addgene_8896)

  • For your References section:

    Degradation of the peroxisome proliferator-activated receptor gamma is linked to ligand-dependent activation. Hauser S, Adelmant G, Sarraf P, Wright HM, Mueller E, Spiegelman BM. J Biol Chem. 2000 Jun 16. 275(24):18527-33. 10.1074/jbc.M001297200 PubMed 10748014
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