|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12307||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5450
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsOneShot-Top10 (Invitrogen)
Copy numberHigh Copy
Alt nameMIC5, SPG1, CAML1, CD171, HSAS1, N-CAML1
SpeciesH. sapiens (human)
Insert Size (bp)3994
Entrez GeneL1CAM (a.k.a. CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:phL1A-pcDNA3 was a gift from Vance Lemmon (Addgene plasmid # 12307 ; http://n2t.net/addgene:12307 ; RRID:Addgene_12307)
For your References section:Molecular structure and functional testing of human L1CAM: an interspecies comparison. Hlavin ML, Lemmon V. Genomics. 1991 Oct . 11(2):416-23. 10.1016/0888-7543(91)90150-D PubMed 1769655