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pPM28 (eroGFP CEN/ARS URA3)
(Plasmid #20131)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20131 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4835
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eroGFP
  • Alt name
    kar2ss-roGFP2-HDEL
  • Alt name
    KAR2
  • Species
    S. cerevisiae (budding yeast); jellyfish
  • Insert Size (bp)
    1828
  • Entrez Gene
    KAR2 (a.k.a. YJL034W, GRP78)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPM28 (eroGFP CEN/ARS URA3) was a gift from Feroz Papa (Addgene plasmid # 20131 ; http://n2t.net/addgene:20131 ; RRID:Addgene_20131)
  • For your References section:

    Real-time redox measurements during endoplasmic reticulum stress reveal interlinked protein folding functions. Merksamer PI, Trusina A, Papa FR. Cell. 2008 Nov 28. 135(5):933-47. 10.1016/j.cell.2008.10.011 PubMed 19026441