Implementation of the CRISPR-Cas9 system in fission yeast.
Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52223 | pMZ222 | Constitutive expression of humanized Cas9 |
| 52224 | pMZ283 | Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments). |
| 52225 | pMZ284 | Expression of sgRNA precursor targeted against ade6+ |
| 52226 | pMZ285 | Expression of sgRNA precursor targeted against ade6M210 |
| 52227 | pMZ286 | Expression of sgRNA precursor targeted against ade6L469 |
| 59896 | pMZ374 | Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments). |
| 59897 | pMZ288 | Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+ |
| 59898 | pMZ289 | Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210 |
| 59899 | pMZ381 | Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469 |