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Implementation of the CRISPR-Cas9 system in fission yeast.
Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. PubMed Article

Plasmids from Article

ID Plasmid Purpose
52223pMZ222Constitutive expression of humanized Cas9
52224pMZ283Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).
52225pMZ284Expression of sgRNA precursor targeted against ade6+
52226pMZ285Expression of sgRNA precursor targeted against ade6M210
52227pMZ286Expression of sgRNA precursor targeted against ade6L469
59896pMZ374Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
59897pMZ288Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+
59898pMZ289Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210
59899pMZ381Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469

Antibodies from Article