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Implementation of the CRISPR-Cas9 system in fission yeast.
Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
52223pMZ222Constitutive expression of humanized Cas9
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52224pMZ283Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).
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52225pMZ284Expression of sgRNA precursor targeted against ade6+
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52226pMZ285Expression of sgRNA precursor targeted against ade6M210
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52227pMZ286Expression of sgRNA precursor targeted against ade6L469
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59896pMZ374Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
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59897pMZ288Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+
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59898pMZ289Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210
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59899pMZ381Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469
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