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(Plasmid #73186)


Item Catalog # Description Quantity Price (USD)
Plasmid 73186 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6833
  • Total vector size (bp) 7355
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    M. musculus (mouse); Glycine max
  • Insert Size (bp)
  • GenBank ID
    NP_082997 AAA61779.1
  • Entrez Gene
    Nphp3 (a.k.a. 3632410F03Rik, AI550417, C230078J01, D330020E01Rik, pcy)
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • internal GFP tag

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

APEX sequence derived from glycine max according to: Rhee et al., Science, 2013

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF5B-FRT-cilia-APEX was a gift from Maxence Nachury (Addgene plasmid # 73186 ; ; RRID:Addgene_73186)
  • For your References section:

    Proteomics of Primary Cilia by Proximity Labeling. Mick DU, Rodrigues RB, Leib RD, Adams CM, Chien AS, Gygi SP, Nachury MV. Dev Cell. 2015 Nov 23;35(4):497-512. doi: 10.1016/j.devcel.2015.10.015. Epub 2015 Nov 12. 10.1016/j.devcel.2015.10.015 PubMed 26585297