5HT6-mCherry-G-GECO1.0
(Plasmid #47500)

• Purpose: Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference
• Depositing Lab: Takanari Inoue
• Publication: Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5950
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: G-GECO1.0
• Species: Synthetic
• Insert Size (bp): 1250
• Tags / Fusion Proteins:
  • 5HT6 (N terminal on insert)
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GAACAGTACGAACGCGCC

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry