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pcDNA3.0-Magneto2.0-p2A-mCherry
(Plasmid #74308)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74308 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 74308-D50 50 μg of DNA in Tris buffer $495
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DNA 74308-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5375
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Magneto2.0
  • Alt name
    TRPV4 (rat); FTL (human); FTH1(human)
  • Species
    H. sapiens (human), R. norvegicus (rat)
  • Insert Size (bp)
    4422
  • Mutation
    TRPV4: delta 760–871
  • Entrez Gene
    FTL (a.k.a. FTL1, LFTD, NBIA3)
  • Entrez Gene
    FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, NBIA9, PIG15, PLIF)
  • Entrez Gene
    Trpv4 (a.k.a. Otrpc4, Vroac)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag
    • p2A-mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.0-Magneto2.0-p2A-mCherry was a gift from Ali Guler (Addgene plasmid # 74308 ; http://n2t.net/addgene:74308 ; RRID:Addgene_74308)

  • For your References section:

    Genetically targeted magnetic control of the nervous system. Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann CD, Guler AD. Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265. 10.1038/nn.4265 PubMed 26950006
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