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PurposeTricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 74309 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.0
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5375
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRPV4-p2A-ferritin-p2A-mCherry
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Alt nameTRPV4 (rat); FTL (human); FTH1(human)
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SpeciesH. sapiens (human), R. norvegicus (rat)
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Insert Size (bp)4815
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Article Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry was a gift from Ali Guler (Addgene plasmid # 74309 ; http://n2t.net/addgene:74309 ; RRID:Addgene_74309) -
For your References section:
Genetically targeted magnetic control of the nervous system. Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann CD, Guler AD. Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265. 10.1038/nn.4265 PubMed 26950006