pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry
(Plasmid #74309)

• Purpose: Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells
• Depositing Lab: Ali Guler
• Publication: Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
• Sequence Information:

  • Depositor Sequences: Full (1)
  • Addgene Sequences: Partial (3)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	74309	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pcDNA3.0
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5375
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRPV4-p2A-ferritin-p2A-mCherry
• Alt name: TRPV4 (rat); FTL (human); FTH1(human)
• Species: H. sapiens (human), R. norvegicus (rat)
• Insert Size (bp): 4815
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry sequence
• Terms and Licenses:
  • UBMTA
  • Clontech Limited Use Label License