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pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA
(Plasmid #74306)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74306 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 3812
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TRPV4
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Trpv4 (a.k.a. Otrpc4, Vroac)
  • Promoter CMV (after cre recombination)
  • Tag / Fusion Protein
    • p2A (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer F1ori-R
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ferritin (FTL and HTL1)
  • Species
    H. sapiens (human)
  • Entrez Gene
    FTL (a.k.a. LFTD, NBIA3)
  • Entrez Gene
    FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, PIG15, PLIF)
  • Tag / Fusion Protein
    • FLAG

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA was a gift from Ali Guler (Addgene plasmid # 74306)
  • For your References section:

    Genetically targeted magnetic control of the nervous system. Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann CD, Guler AD. Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265. 10.1038/nn.4265 PubMed 26950006