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Plasmid#155339PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-iCre-ERT2
Plasmid#137858PurposeCAG-iCre-ERT2 piggyBac transposonDepositorInsertiCre-ERT2
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-DDRGK1-WT-HA
Plasmid#139845PurposeLentiviral expression of human DDRGK1-WT-HADepositorInsertDDRGK1 (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
hRIP3 GFP wt
Plasmid#41387DepositorInsertRIP3 (RIPK3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cSrc
Plasmid#214233PurposeBacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertcSrc Kinase domain (SRC Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Sm
Plasmid#45937Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneUseTags6xHis and Twin-StrepExpressionBacterialMutationPromoterTrc (lactose/IPTG inducible)Available sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFast-hHus1
Plasmid#222469PurposeExpresses hHus1 in insect cellsDepositorInserthHus1 (HUS1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag Rac2
Plasmid#12192DepositorInsertRac2 (RAC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFast-Flag-hHus1
Plasmid#222470PurposeExpresses hHus1 in insect cellsDepositorInserthHus1 (HUS1 Human)
UseTagsFlag epitopeExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFast-His6-hRad17
Plasmid#222465PurposeExpresses hRad17 in insect cellsDepositorInserthRad17 (RAD17 Human)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETB PKA RIalpha
Plasmid#14922DepositorInsertPKA R1alpha regulatory subunit (PRKAR1A Bovine)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPET-PKR/PPase
Plasmid#42934DepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCBL101-RUBY
Plasmid#199723PurposeExpresses betalain biosynthesis genes under CaMV 35S promoterDepositorInsert35S-RUBY
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HDAC6.ΔBuz-FLAG
Plasmid#30484DepositorInsertHDAC6 (HDAC6 Human)
UseTagsFLAGExpressionMammalianMutationDeletion of the C-terminal Ubiquitin binding doma…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP2D6
Plasmid#67090PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP2D6 (CYP2D6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro-cOVA
Plasmid#135073PurposeExpresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistanceDepositorInsertcOVA (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI NL4-3 Nef-HA-WT
Plasmid#24162DepositorInsertNef (nef HIV)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
Plasmid#179105PurposepCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINTDepositorInsertpCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mNeonGreen
Plasmid#98882PurposeIn vivo visualization of lysosomes (can be used for colocalization studies)DepositorArticleInsertLamp1
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-SUMO-MarathonRT
Plasmid#109029PurposeGroup II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”DepositorInsertERE_01490
UseTags6xHis-SUMO tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only