164,247 results

1. PB-SAM

   Plasmid

   #102559

   Purpose

   Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Inserts

   MS2-P65-HSF1_T2A_Hygro
   dCAS9(D10A, N863A)-VP64_T2A_Blast
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9 and N55K in MS2

   Promoter

   CAG and EF1A

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3EF1aH-myr-Akt

   Plasmid

   #179909

   Purpose

   This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

   Depositor

   Xin Chen

   Article

   Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-LMP1-IRES-GFP

   Plasmid

   #220350

   Purpose

   Expresses EBV latent gene LMP1 in mammalian cells

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS130

   Plasmid

   #26290

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Drosophila transgenesis

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. EphA2-YFP

   Plasmid

   #108852

   Purpose

   Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

   Depositor

   Kalina Hristova

   Article

   Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

   Insert

   EPHA2 (EPHA2 Human)
   

   Use

   Tags

   Labeled with eYFP on the C-Terminus via a 15 amin…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-MRLC1

   Plasmid

   #35680

   Depositor

   Tom Egelhoff

   Article

   Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pET15b-RvLEAMshort

   Plasmid

   #218122

   Purpose

   Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

   Depositor

   Ci Ji Lim

   Article

   Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

   Insert

   RvLEAMshort
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Truncated form (58-181aa)

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC1 Grx1-roCherry-mito

   Plasmid

   #141069

   Purpose

   Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

   Depositor

   Dmitry Bilan

   Article

   Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

   Insert

   Grx1-roCherry
   

   Use

   Tags

   dMTS

   Expression

   Mammalian

   Mutation

   Changed Alanine 150 to Cysteine, inserted Threoni…

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIP403

   Plasmid

   #122028

   Purpose

   Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

   Depositor

   Lars Axelsson, Geir Mathiesen

   Article

   Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

   Insert

   gusA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sppA

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-VSVG

    Plasmid

    #11912

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F64L, S65T, A206K relative the wild type GFP sequ…

    Promoter

    Available Since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP‐RNASEH2A

    Plasmid

    #108700

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2A (RNASEH2A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-Beclin 1

    Plasmid

    #86748

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    human Beclin 1 (BECN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNPTS138 Cm

    Plasmid

    #41891

    Depositor

    Howard Steinman

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCK054 pDEST exorh:mCerulean

    Plasmid

    #195985

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFPC1-EPSIN 1

    Plasmid

    #22228

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    please see depositor comment below

    Promoter

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-FLEX-FLIM-AKAR

    Plasmid

    #60445

    Purpose

    Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKAR
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMU-NUCr

    Plasmid

    #61168

    Purpose

    Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    NLS-mCherry-GUS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFD116

    Plasmid

    #124769

    Purpose

    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    dcas9
    PpflB sgRNA BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE-Lepr_FL-IRES-eGFP

    Plasmid

    #196635

    Purpose

    Doxycycline inducible expression of full length mouse Lepr with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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