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pCMV5B-Flag-Smurf1 C699A
(Plasmid #11753)

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Item Catalog # Description Quantity Price (USD)
Plasmid 11753 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 11753-D50 50 μg of DNA in Tris buffer $495 *
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DNA 11753-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pCMV5B
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMURF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Mutation
    C699A: catalytically inactive
  • GenBank ID
    NM_020429
  • Entrez Gene
    SMURF1
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A human Smurf1 cDNA encoding all but the first eight amino acids was identified in the expressed-sequence-tag database (AA292123), and full-length hSmurf1 was reconstituted using a fragment of XSmurf1 cDNA encoding the first eight amino acids.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5B-Flag-Smurf1 C699A was a gift from Jeff Wrana (Addgene plasmid # 11753 ; http://n2t.net/addgene:11753 ; RRID:Addgene_11753)

  • For your References section:

    A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Zhu H, Kavsak P, Abdollah S, Wrana JL, Thomsen GH. Nature. 1999 Aug 12. 400(6745):687-93. 10.1038/23293 PubMed 10458166
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