165,550 results

1. pHCMV_DENV3_PrME

   Plasmid

   #207265

   Purpose

   Mammalian expression of Dengue virus 3 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV3_PrME (POLY Dengue virus 3)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCXB-EBNA1

   Plasmid

   #41857

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   EBNA-1 (EBNA-1 human herpesvirus 4)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCS-13X Linker-BioID2-HA

   Plasmid

   #80899

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

   Plasmid

   #100146

   Purpose

   Expresses CPT1A

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   CPT1A (CPT1A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP-KIF5B-Rigor

   Plasmid

   #172204

   Purpose

   Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

   Insert

   KIF5B (Kif5b Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVXT Sox4

   Plasmid

   #101121

   Purpose

   Sox4 coding region

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   SOX4 (Sox4 Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-hTimeless (pc4729)

   Plasmid

   #216861

   Purpose

   Mammalian expression vector encoding GFP tagged human Timeless

   Depositor

   Cristina Cardoso

   Article

   Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

   Insert

   Timeless (TIMELESS Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYFAC-CH2-ubi-Y-pyrG

   Plasmid

   #221131

   Purpose

   Expression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

   Type

   Empty backbone

   Use

   Synthetic Biology; Expression in aspergillus and …

   Promoter

   PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBB542

   Plasmid

   #27395

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   dnaK, dnaJ, groESL
   

   Expression

   Bacterial

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-f:PGC1

    Plasmid

    #1026

    Depositor

    Bruce Spiegelman

    Article

    Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

    Insert

    PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-MED17

    Plasmid

    #15410

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED17 (MED17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-gp32-H6

    Plasmid

    #163912

    Purpose

    Expresses T4 gp32 for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    gp32
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. prhaBAD-anti-mouse-Nb-Hia5

    Plasmid

    #239626

    Purpose

    Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

    Depositor

    Aaron Straight

    Article

    Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

    Insert

    Hia5
    

    Tags

    6HIS, MBP, TEV, and anti-Mouse Nanobody

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available Since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PGK-DTAG-NTERM-GFP

    Plasmid

    #185775

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    William Sellers, Alessandra Ianari

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1α1.1-GFP

    Plasmid

    #122100

    Purpose

    AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV2

    Insert

    GFP
    

    Use

    AAV

    Tags

    NA

    Expression

    Mammalian

    Promoter

    EF1a（1.1kb short version)

    Available Since

    May 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.GFAP.iGABASnFR

    Plasmid

    #112172

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR
    

    Use

    AAV

    Promoter

    GFAP

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSET Nucleolin

    Plasmid

    #13037

    Depositor

    Peter Newburger

    Article

    Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

    Insert

    Nucleolin (NCL Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. trLATmEos3.2

    Plasmid

    #203745

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLAT (LAT Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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