pBabe-puro GSK3 beta (S9A)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14128||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAvailable at Addgene (#1764)
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGSK3 beta (S9A)
SpeciesH. sapiens (human)
Insert Size (bp)1300
MutationS9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
/ Fusion Protein
- V5 tag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBabe 5' (Common Sequencing Primers)
pBabe-puro-GSK3β-S9A was generated by standard site-directed mutagenesis from a cDNA clone of human GSK3β.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe-puro GSK3 beta (S9A) was a gift from Brendan Manning (Addgene plasmid # 14128 ; http://n2t.net/addgene:14128 ; RRID:Addgene_14128)
For your References section:S6K1 regulates GSK3 under conditions of mTOR-dependent feedback inhibition of Akt. Zhang HH, Lipovsky AI, Dibble CC, Sahin M, Manning BD. Mol Cell. 2006 Oct 20. 24(2):185-97. 10.1016/j.molcel.2006.09.019 PubMed 17052453