pBabe-puro GSK3 beta (S9A)
(Plasmid #14128)

• Depositing Lab: Brendan Manning
• Publication: Zhang et al Mol Cell. 2006 Oct 20. 24(2):185-97.

Backbone

• Vector backbone: pBabe-puro
• Backbone manufacturer: Available at Addgene (#1764)
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GSK3 beta (S9A)
• Alt name: GSK3b
• Alt name: GSK3beta
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: S9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
• Entrez Gene: GSK3B
• Tag / Fusion Protein:
  • V5 tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBabe 5'

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBabe-puro-GSK3β-S9A was generated by standard site-directed mutagenesis from a cDNA clone of human GSK3β.