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pBabe-puro GSK3 beta (S9A)
(Plasmid #14128)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14128 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-puro
  • Backbone manufacturer
    Available at Addgene (#1764)
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSK3 beta (S9A)
  • Alt name
    GSK3b
  • Alt name
    GSK3beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    S9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
  • Entrez Gene
    GSK3B
  • Tag / Fusion Protein
    • V5 tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBabe 5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

pBabe-puro-GSK3β-S9A was generated by standard site-directed mutagenesis from a cDNA clone of human GSK3β.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-puro GSK3 beta (S9A) was a gift from Brendan Manning (Addgene plasmid # 14128 ; http://n2t.net/addgene:14128 ; RRID:Addgene_14128)
  • For your References section:

    S6K1 regulates GSK3 under conditions of mTOR-dependent feedback inhibition of Akt. Zhang HH, Lipovsky AI, Dibble CC, Sahin M, Manning BD. Mol Cell. 2006 Oct 20. 24(2):185-97. 10.1016/j.molcel.2006.09.019 PubMed 17052453