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Depositing Lab
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Depositing OrganizationBrigham and Women's Hospital
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13466 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 13466-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 13466-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepIRESpuro3
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHMGA1
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SpeciesH. sapiens (human)
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Insert Size (bp)323
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GenBank IDNM_145899.2
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Entrez GeneHMGA1 (a.k.a. HMG-R, HMGA1A, HMGIY)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV forward
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byIMAGE Consortium
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The HMGA1 coding sequence was PCR amplified from IMAGE clone 5399570 (Genbank accession no. BC063434) using gene-specific primers modified to include the appropriate restriction sites at their 5' end. The primers used were forward, 5'-TTTTGATATCATGAGTGAGTCGAGCTCGAAG-3' and backward, 5'-TTTTGAATTCTCACTGCTCCTCCTCCGAGGA-3'. Purified PCR products were digested with EcoRV and EcoRI before ligation into an EcoRV/EcoRI–digested pIRES-puro3 vector (Clontech, Palo Alto, CA).
DNA (Catalog # 13466-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 13466-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRES-HMGA1 was a gift from Edward Whang (Addgene plasmid # 13466 ; http://n2t.net/addgene:13466 ; RRID:Addgene_13466) -
For your References section:
HMGA1 is a determinant of cellular invasiveness and in vivo metastatic potential in pancreatic adenocarcinoma. Liau SS, Jazag A, Whang EE. Cancer Res. 2006 Dec 15. 66(24):11613-22. 10.1158/0008-5472.CAN-06-1460 PubMed 17178855
